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Энергетический баланс и чистая эффективность роста плоской устрицы (Ostrea edulis L.) IBSS Repository
Золотницкий, А. П.; Сытник, Н. А..
Исследованы энергетический баланс и чистая эффективность роста (коэффициент К2) плоской устрицы (Ostrea edulis L.) в онтогенезе. Выявлены общие тенденции изменений отдельных его компонентов в процессе роста и распределение энергетических трат на индивидуальную продукцию, энергетический обмен, ассимилированную и потреблённую пищу. Дана характеристика сезонных изменений чистой эффективности роста, связанных с температурой воды и массой тела, динамика которых описывается уравнением множественной регрессии. Показано, что максимальные значения К2 у исследованного вида могут составлять 0.63 – 0.67.
Tipo: Journal Contribution Palavras-chave: Устрица; Энергетический баланс; Рост; Метаболизм; Ассимиляция; Рацион; Эффективность; Устриця; Енергетичний баланс; Зростання; Метаболізм; Асиміляція; Раціон; Ефективність; Oyster; Energy balance; Growth; Metabolism; Assimilation; Ration; Efficiency.
Ano: 2010 URL: http://repository.ibss.org.ua/dspace/handle/99011/1919
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Open-air treatment of wastewater from land-based marine fish farms in extensive and intensive systems: current technology and future perspectives ArchiMer
Hussenot, Jerome; Lefebvre, Sébastien; Brossard, Nicolas.
Marine land-based fish farms located in coastal wetlands (salt-pond zones, lagoon banks, etc.), whether extensive or intensive, send farm effluents directly to the sea or after short periods of stocking in retention reservoirs. The aims of our investigation have been to compare the efficiency of current and potential water treatment procedures in open-air. Wastewater retention ponds in commercial farms (Atlantic coasts of France) are efficient in removing up to 1 metric ton of particulate material (dry weight) per hectare and per day (faeces and unconsumed feed), but are inefficient in reducing dissolved wastes, both organic (urea, amino acids, protein) and inorganic (total ammonia nitrogen, phosphates). Forthcoming outdoor technology to treat these forms...
Tipo: Text Palavras-chave: Traitement de l'eau; Effluent; Décantation; Sédimentation; Écumage; Microalgue; Huître; Water treatment; Effluent; Aquaculture; Sedimentation; Foam fractionation; Microalgae; Oyster.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/1998/publication-873.pdf
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Use of an ecosystem model for the assessment of the influence of cultivated oysters (Crassostrea gigas) on the nitrogen cycling in the Marennes-Oleron Bay ArchiMer
Bacher, Cedric.
A model of the growth of cultivated oyster in the Marennes-Oléron bay was developped to assess the influence of the nutrients input and of the amount of oysters on the growth performance (Railiard. 1991). The ecosystem main characteristics are: - short residence time of the water. - high turbidity level, depending on the season, tidal level, bathymetry, currents and wind. The turbidity acts on the primary production through light limitation and on oyster production as a food dilution factor. - presence of high density cultivated areas on mid-tidal flats. The amount of oysters is ranging between 80 000 and 110 000 tons according to the year. - spatial variability of the biological and physical features. - coupling effects of the physic process (transport,...
Tipo: Text Palavras-chave: Ecosystem model; Oyster; Crassostrea gigas; Nitrogen cycling; Marennes Oleron Bay; Charente Maritime.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00102/21300/18914.pdf
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Rapid and sensitive detection of ostreid Herpesvirus 1 in oyster samples by real-time PCR (Poster) ArchiMer
Pepin, Jean-francois; Riou, Antoine; Renault, Tristan.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region, which is present in two copies in the OsHV-1 genome and encodes two proteins of unknown function. The assay was applied successfully to rapid diagnosis (100 minutes)...
Tipo: Text Palavras-chave: Crassostrea gigas; PCR; Oyster; OsHV 1; Detection; Herpes virus 1.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3653.pdf
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Feeding of Crassostrea gigas at high seston load : modeling requirements ArchiMer
Barille, Laurent; Prou, Jean.
Ecophysiological experiments conducted in situ in the Bay of Marennes-Oléron during a spring and neap tide cycle in May 1991 , were designed to get a better representation of 2 important components of the energy budget of the Japanese oyster Crassostrea gigas : clearance rate and selection efficiency. The relationships previously established by Raillard et al. ( 1993) are re-evaluated by integrating the effects of high seston loads, typical of tidal effect within estuaries, on the physiological processes. For the clearance rate, the re-evaluated parameterization includes: - a negative effect of turbidity. - a clogging threshold set to 192 mg/l. Selection efficiency parameterizations are modified by incorporating : - a constant inorganic ingestion for...
Tipo: Text Palavras-chave: Matematical model; Oyster; Crassostrea gigas; Clearance rate; Seclection efficency; Marennes Oleron; Charente Maritime.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00102/21299/18913.pdf
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Gonad volume assessment in the oyster Crassostrea gigas: Comparison between a histological method and a Magnetic Resonance Imaging (MRI) method ArchiMer
Flahauw, Emilie; Quellec, Stephane; Davenel, Armel; Degremont, Lionel; Lapegue, Sylvie; Hatt, Philippe-jacques.
Previous studies comparing Crassostrea gigas selected lines with different susceptibility to summer mortality concluded that the higher the reproductive effort the lower the survival. But theywere lacking individual followup of this reproductive effort which would be of great interest to help us to understand the physiology of the oysters facing summermortalities. Themost frequently usedmethod for assessing the volume of the gonad is to measure the area it occupies on histological sections. Gonad measurement by magnetic resonance imaging, in contrast, is non-destructive and therefore makes it possible to assess individual evolution as well as to compare individuals. The present study compared the values of gonad volume by thismethodwith the values of gonad...
Tipo: Text Palavras-chave: Oyster; Crassostrea gigas; Reproduction; Histology; Magnetic resonance imaging; MRI.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00102/21309/19410.pdf
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Performances de croissance de l'huître creuse, Crassostrea gigas (Thunberg) dans le bassin d'élevage de Marennes-Oléron entre 1979 & 1982 ArchiMer
Berthome, Jean-paul; Prou, Jean; Bodoy, Alain.
Growth of the japanese oyster, Crassostrea gigas was monitored during 4 years, by following the recruitment of the year 1978, held in rearing conditions. After three and half years, the average length was la cm and the average total weight was 80 g. Clear seasonal changes were observed on the growth in length and weight. An important loss in dry weight occured in July, during the release of gametes. Such losses constituted 29 %, 43 % and 60 % of the dry weight, respectively for individuals which were 2 years, 3 years and 4 years old. The higher instantaneous growth rates (G) were observed during spring and at the beginning of summer. Growth model of Von Bertalanffy, gave the best estimate for the length growth of the species. During the 1979-1982...
Tipo: Text Palavras-chave: Growth parameter; Growth model; Reardd stock; Crassostrea gigas; Oyster; Growth; Descripteurs de croissance; Modèle de croissance; Stock cultivé; Crassostrea gigas; Huître; Croissance.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/publication-2967.pdf
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Real-time PCR assay for rapid detection and quantification of Vibrio aestuarianus in oyster and seawater: A useful tool for epidemiologic studies ArchiMer
Saulnier, Denis; De Decker, Sophie; Haffner, Philippe.
Because Vibrio aestuarianus is known to cause serious infections in Pacific oyster Crassostrea gigas, a real-time PCR assay was developed targeting the dnaJ gene of this bacterium. Only V. aestuarianus strains isolated from C. gigas mortality events in different geographic areas and the reference strain tested positive, whereas no amplification products was obtained with type strains belonging to 23 other species of Vibrio. Sensitivity and reproducibility of the method were assessed using either seawater or oyster homogenate samples spiked with one V aestuarianus strain. All these samples were stored at -20 degrees C in order to mimic retrospective or grouped natural sample analysis without quantification bias due to prolonged freezing. Analysis of...
Tipo: Text Palavras-chave: V. aestuarianus; Vibrio; Taqman; Real time PCR; Pathogen; Oyster; Crassostrea gigas.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6447.pdf
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Dynamique des sédiments fins et mixtes des zones intertidales de la baie de Marennes-Oléron ArchiMer
Kervella, Stephane.
This thesis aims at increasing the general knowledge on sediment dynamics of intertidal flats in the Marennes-Oléron Bay, according to physical, biological and anthropogenic factors. The conducted research is compound of three complementary studies: (1) a study of sediment behaviour through applied rheophysics on intertidal superficial sediments; (2) a hydro-sedimentary study based on identification of the forcing action (physical, biological or anthropogenic) on the intertidal flat stability over several time scales (from the event to several months); (3) a specific application of the model SiAM-2DH to the Marennes-Oléron Bay. The surface sediments shows a mechanical behaviour that mainly depends on the fine fraction for a composition that contains up...
Tipo: Text Palavras-chave: Rhéophysique; Seuil d’écoulement; Sédiments cohésifs et mixtes; Sédimentation; Érosion; Hydrodynamique; Vasière; Zone intertidale; Conchyliculture; ALTUS; SiAM-2DH; Baie de Marennes-Oléron; Rhéophysics; Shear strenght; Cohesive and mixed sediment; Sédimentation; Erosion; Hydrodynamics; Mudflat; Intertidal area; Oyster; ALTUS; SiAM-2DH; Marennes-Oléron Bay..
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00004/11534/8117.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf
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Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption ArchiMer
Le Guyader, Soizick; Bon, Fabienne; Demedici, Dario; Parnaudeau, Sylvain; Bertone, Alessandra; Crudeli, Silvia; Doyle, Aoife; Zidane, Mohamed; Suffredini, Elisabetta; Kohli E, Evelyne; Maddalo, Francesco; Monini, Marina; Gallay, Anne; Pommepuy, Monique; Pothier, Pierre; Ruggeri, Franco M..
An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to...
Tipo: Text Palavras-chave: Seafood; Contamination; Norovirus; Epidemiology; Virology; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2096.pdf
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Traditional oyster culture in France ArchiMer
Heral, Maurice.
The indigenous oyster of mainland France, the fiat oyster, Ostrea edulis has been part of the human diet for centuries. The Romans collected them and exported them to Rome. Although tanks for holding oysters after harvesting were in use at that time (GreIon 1978) it seems that true culture was not developed along the coast contrary to the records of Pliny the Older. It appears that oysters were already being captured on hoards off the Italian coast. The exploitation of natural stocks continued through the Middle Ages and the Renaissance. However, it was not until the 17th century that oyster culture began, first in the pools of the salt marshes of the Atlantic coast and then in specially managed ponds. Papy (1941) repeats a good description given in 1688...
Tipo: Text Palavras-chave: Rearing; Diseases; Analytical model; Overall model; Cultured stocks; Energetics; Reproduction; Nutrition; Physiology; France; Culture; Oyster.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/publication-3039.pdf
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Modulations of the interactions between pacific cupped oyster Crassostrea gigas and Vibrio according to bacterial virulence and to genetic and physiological status of the host : Methodology and first results ArchiMer
De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis.
Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and...
Tipo: Text Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf
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Cryopreservation of Crassostrea gigas vesicular cells: Viability and metabolic activity ArchiMer
Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m..
Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into...
Tipo: Text Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf
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Characterization of a gonad-specific transforming growth factor-beta superfamily member differentially expressed during the reproductive cycle of the oyster Crassostrea gigas ArchiMer
Fleury, Elodie; Fabioux, Caroline; Lelong, C; Favrel, P; Huvet, Arnaud.
Through differential screening between oyster families selected for high and low summer survival, we have characterized a new transforming growth factor-beta (TGF-beta) superfamily member. This novel factor, named oyster-gonadal-TGF beta-like (og-TGF beta-like), is synthesized as a 307 amino acid precursor and displays 6 of the 7 characteristic cysteine residues of the C-terminal, mature peptide. Sequence comparison revealed that og-TGF beta-like has a low percentage of identity with other known TGF-beta superfamily members, suggesting that og-TGF beta-like is a derived member of this large superfamily. Real-time PCR (RT-PCR) analysis in different oyster tissues showed that og-TGF beta-like is specifically expressed in both male and female gonads, at...
Tipo: Text Palavras-chave: Reproduction; Oyster; Gene expression; Transforming growth factor; Bivalve mollusc.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3899.pdf
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Ingestion of a bacterivorous ciliate by the oyster Crassostrea gigas: Protozoa as a trophic link between picoplankton and benthic suspension-feeders ArchiMer
Le Gall, Solange; Bel Hassen, Malika; Le Gall, Pierre.
The linked concepts of 'microbial loop' and 'protozoan trophic link' have been very well documented in filter-feeding microzooplankton such as copepods, but have not been applied to energy transfer to benthic suspension-feeding macrofauna, with the exception of the recent demonstration of heterotrophic flagellate assimilation by mussels. The oyster Crassostrea gigas obtains energy resources by filtering microalgae (similar to 5 to 100 mu m). However, in turbid estuaries, light-limited phytoplanktonic production cannot entirely account for oyster energy requirements. Conversely, picoplankters (<2 mu m), which are main effecters of coastal energy flow and matter cycling, are not efficiently retained by oyster filtration. Ciliate protozoal as both...
Tipo: Text Palavras-chave: Oyster; Food sources; Picoplankton; Protozoa; Trophic link.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00337/44788/44482.pdf
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Etude comparative des caractéristiques écophysiologiques et des performances de croissance de l'huître creuse Crassostrea gigas diploïde et triploïde en milieu contrôlé ArchiMer
Haure, Joel; Fortin, Adeline; Dupuy, Beatrice; Nourry, Max; Palvadeau, Hubert; Papin, Mathias; Penisson, Christian; Martin, Jean-louis.
Introduction: Marine farming in France is currently very widely dominated by oyster farming. French oyster production totals 148,467 tons, for a turnover of close to 280 million euros, making France the leading European producer. The two main species concerned are the cup oyster, Crassostrea gigas (140,000 tons) and the flat oyster, Ostrea edulis (2,000 tons), the production of which has collapsed since 1970 following successive epizootics of Marteilia refringens and Bonamia ostreae. French oyster farming is thus in a situation of quasi single-crop farming, with more than 90% of tonnage pertaining to the cup oyster (FAO data from 1993). This situation of quasi single-crop farming leads to the pessimistic observation that one hypothetical crisis in the...
Tipo: Text Palavras-chave: Growth; Ecophysiology; Oyster; Crassostrea Gigas; Eeproduction; Milieu Controle; Croissance; Triploide; Diploide; Ostreidae; Huitre; Crassostrea Gigas; Ecophysiologie; Reproduction.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/rapport-1509.pdf
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Overwiew on selective breeding and genetic improvement in bivalve shellfish ArchiMer
Boudry, Pierre.
Dr. Boudry focussed his talk on oysters, which are the most important bivalve species produced world wide (more than 4 million tons). One of the main characteristic of bivalve aquaculture is that most of the juveniles ("seed or "spat") are collected from natural recruitment (e.g. 100% of the European mussel production). Hatchery propagation is used in the case of introduced species (e.g. Crassostrea gigas in USA) or limited natural recruitment. The most significant genetic improvement for the production of Pacific oyster (C. gigas) to date has been obtained through the breeding of triploids, especially since the development of tetraploids. Triploidy lead to highly reduced gametogenesis and, as a result better growth and survival. Many quantitative...
Tipo: Text Palavras-chave: Crassostrea gigas; Oyster; Genetic variability; Bivalve shellfish; Selective breeding; Genetic.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3479.pdf
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Effect of orgnotin compounds (TBT) used in antifouling paints on cultured marine molluscs - a literature study ArchiMer
Heral, Maurice; Alzieu, Claude; Deslous-paoli, Jean-marc.
Organotin salts, used as antifouling substances have a deleterious effect on the environment particularly on molluscs and especially on the Japanese oyster Crassostrea gigas. A synthesis of the effects of tributyltin (TBT) on cultured molluscs reveals the following effects: 1) acute and chronic toxicity to the adults; 2) accumulation in the flesh 01 the adults; 3) perturbation 01 reproduction particularly fertility, mortality 01 offspring and decrease in larval growth rate; 4) decrease in the growth rate 01 juveniles and thickening 01 the shell; and 5) modification 01 the physiology al sublethal levels.
Tipo: Text Palavras-chave: TBT; Crassostrea gigas; Oyster; Marine molluscs; Organotin.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/publication-3016.pdf
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Growth of the Pacific oyster (Crassostrea gigas) in a high-turbidity environment: Comparison of model simulations based on scope for growth and dynamic energy budgets ArchiMer
Barille, Laurent; Lerouxel, Astrid; Dutertre, Mickael; Haure, Joel; Barille, Anne-laure; Pouvreau, Stephane; Alunno-bruscia, Marianne.
We compared growth simulations by dynamic energy budget (DEB) and scope for growth (SFG) models of the Pacific oyster Crassostrea gigas, cultivated in Bourgneuf Bay on the French Atlantic coast. This bay is located at a latitude in the middle of the European range of the species, and is characterized by high concentrations of suspended particulate matter (SPM) and a marked gradient between high-turbidity sites in the north (daily SPM > 500 mg L-1) and intermediate-turbidity sites in the south. The models use two forcing variables: seawater temperature and food density. We tested two indices of food availability: chlorophyll a and microalgal concentrations. In the SFG model, food intake is simulated by a type-II Holling functional response, as in the DEB...
Tipo: Text Palavras-chave: Oyster; Dynamic Energy Budget model; Scope for growth model; Turbidity.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15433/12783.pdf
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